By Christophe Clement, Ettore Pacini, Jean-Claude Audran
Pollen, the plant constitution most generally utilized by people, is a key constitution in plant replica giving upward push to culmination and seeds. additionally, the biotechnological use of pollen is of significant significance for plant breeders because it permits to acquire types with greater usage and yield. within the first half, the successive steps of pollen improvement within the anther from floral induction to pollen germination and fertilization are completely tested; the second one half is dedicated to pollen behaviour in vitro.
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Additional resources for Anther and Pollen: From Biology to Biotechnology
_ •• ~ . _ ..... ,01'\ I. ,) t\ SO 100 ,1 - .. ISO '\ _.. ~ • _ .. - :-<10"'- 200 •• _ - ... ~ - • 250 300 350 400 4SO pillel position Fig. 3. Densitometric analysis of ubiquitin-protein conjugates in microgametophytes from male (unbroken line) and female (dotted line) flowers of kiwifruit during development. 15 /-lg of protein extracts were fractionated on an SDSI13% polyacrylamide gel and immunoblotted as described in fig. 2. A: day 14 (tetrads); B: day 21 (early-uninucleate microspore); C: day 29 (male: early-binucleate pollen; female: degenerating pollen at the uninucleate stage); D: day 35 (male: mature pollen; female: degenerated pollen).
F. Liang and A. R. Ferguson growing in experimental plots near Faenza (Italy) were sampled during flower development starting from buds 1 cm in diameter (day 0). Either whole anthers or isolated microgametophytes of male and female flowers from the microspore tetrad stage (day 14) to mature pollen (day 35) were extracted in 50 mM Tris-HCl buffer pH 8 containing protease inhibitors. Protein content was determined according to Lowry et al. (1951) after TCA precipitation. SDSPAGE was carried out according to Laemmli (1970).
Pellets were washed twice with 5% TeA and resuspended in TeA. 36 Biasi et aI. Three replicates of supernatant and pellet were hydrolysed with the same volume of 12 N HCl for 12 h at 110°C in flame sealed ampoules according to Slocum and Galston (1985). Following centrifugation at low speed to remove charred debris, the hydrolysates were taken to dryness under vacuum in a 60°C bath and resuspended in 200 III of TCA. Replicates of both supernatant and hydrolysate were dansylated as described by Smith and Best (1977).